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Genotoxicity

Genotoxicity

Genotoxicity refers to the destructive effects of mutagens such as high energy radiation and chemicals on cellular nucleic acids, particularly nuclear DNA. Single cell gel electrophoresis, or the comet assay, can be used to probe DNA fracture by mutagens and the g-H2AX immunofluorescence assay can used to quantify DNA double strand breaks. Each method has its challenges with respect to image analysis, but Gen5 3.0 has the ability to quantify either the %DNA in the comet tail or comet tail moment; or count g-H2AX foci to determine levels of genotoxicity.

 

Application Notes:

 

Related Webinars:

 

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Learn more about Genotoxicity applications - search our Application Notes and technical documentation in Resources.

 

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