Genotoxicity

GenotoxicityGenotoxicity refers to the destructive effects of mutagens such as high energy radiation and chemicals on cellular nucleic acids, particularly nuclear DNA. Single cell gel electrophoresis, or the Comet Assay, can be used to probe DNA fracture by mutagens. The g-H2AX immunofluorescence assay can be used to quantify DNA double strand breaks. Each method has its challenges regarding image analysis, but Agilent BioTek Gen5 data analysis software 3.0 has the ability to quantify either the %DNA in the comet tail or comet tail moment. The Gen5 3.0 can also count g-H2AX foci to determine levels of genotoxicity.

 

 

 

Application Notes:

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Visual Abstracts:

Agilent BioTek visual abstracts are brief animated presentations describing the augmented microscopy workflow of a specific application.

H2AX FOCI SPOT COUNTING AS A DETERMINATION OF GENOTOXICITY
γH2AX Foci Spot Counting as a Determination of Genotoxicity

Comet Assay: Single Cell Genotoxicity Measurement
Comet Assay: Single Cell Genotoxicity Measurement

 

 

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Learn more about Genotoxicity applications - search our application notes and technical documentation in Resources.

 

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