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Confluence

 

Confluence measurements provide valuable information on the extent that cell populations have changed over time. This measurement is routinely determined prior to splitting cells in tissue culture or for assays determining cell proliferation where cell counting may be problematic (i.e. cell types that tend to clump). BioTek’s Lionheart FX and Cytation imaging systems, along with Gen5 software, can automate the analysis across any number of labware, from culture flasks, petri dishes and low density microplates (6- and 12-well microplates) suitable for tissue culture to higher density microplates used for cell-based assays.

 

Cells imaged with high contrast brightfield
27% confluence
Gen5 object masks identifies cells for confluence determination
43% confluence
Proliferative or anti-proliferative pharmacology is graphed
62% confluence
Proliferative or anti-proliferative pharmacology is graphed
85% confluence

 

Cells imaged with high contrast brightfield
Gen5 object masks identifies cells for confluence determination
Proliferative or anti-proliferative pharmacology is graphed
 
Proliferative or anti-proliferative pharmacology is graphed

 

Application Notes:

 

Related Applications:

 

Visual Abstracts (.mp4):

BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.

Cell Proliferation
Cell Proliferation - Label-free Confluence

 
 

 

Related Products:

Learn more about Confluence applications - search our Application Notes and technical documentation in Resources.

 

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