Live Cell Assays
Kinetic live cell assays provide real-time data of cellular responses. BioTek instruments enable a wide variety of live cell applications addressing diverse biological processes, ranging in timescales from milliseconds to multiple days.
Studies involving fast-acting cellular responses, including Ca2+ mobilization in response to GPCR activation, require the ability to capture events with sub-second resolution. Available inline reagent injectors and rapid image capture rates support uninterrupted monitoring of cells and provide detailed characterization of events.
Quantifying cell viability and growth rates is a crucial aspect of biological research and therapeutic drug development. Complete environmental control, including CO2, O2, temperature, and humidity, ensures optimal conditions for conducting long-term kinetic cell viability and proliferation assays.
The following application notes provide a sampling of the live cell assays that were optimized on BioTek microplate readers and automated imaging systems for robust and reproducible results.
- Characterizing Calcium Mobilization using Kinetic Live Cell Imaging
- Kinetic Proliferation Assay Using Label-Free Cell Counting
- Live Cell Hypoxia Determination in a 3D Spheroid Model
- Performance of a Label-Free Image-Based 2D Scratch Wound Healing Assay to monitor Cell Migration and its Inhibition