Synergy 2 Multi-Mode Microplate Reader

Detection > Filter-based Multi-Mode Microplate Readers

Overview:

The Synergy 2 is BioTek's high-performance multi-mode plate reader designed for the life science research and drug discovery markets. Its fluorescence detection system uses deep blocking filters and dichroic mirrors for the best level of performance in research and screening applications. The dedicated absorbance detection system is monochromator-based, providing the best level of flexibility in this read mode. And, by utilizing dedicated optical elements for each individual detection technique, Synergy 2 offers uncompromised performance and the lowest limits of detection.

Detection modes include Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence, AlphaScreen, Luminescence and UV-visible Absorbance. Its three broad-spectrum light sources have been chosen for optimal illumination and excitation in all applications.

The Synergy 2 is controlled by BioTek's Gen5™ Reader Control and Data Analysis Software, is certified for HTRF®, DLR™ and LanthaScreen™, and is compatible with AlphaScreen assays.

US Federal Government customers:

This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

HTRF® and the HTRF logo are registered trademarks of Cisbio.
Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark office.
AlphaScreen® is a registered trademark of Perkin Elmer.
Transcreener® is a registered trademark of BellBrook Labs.
The LanthaScreen™ and GeneBLAzer® technologies from Invitrogen have been validated on BioTek instruments.

Features:

Measurement Techniques: Fluorescense Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, Glow and Flash Luminescence, UV-Vis Absorbance, FRET, TR-FRET, BRET, Area Scanning, Spectral Scanning
Unique Optical System: a narrow band monochromator, optimized filter/dichroic mirror pairing, and three broad spectrum light sources achieve superior performance in all read modes
Compatible with Take3 Multi-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
High Sensitivity: dedicated optical elements optimized for each specific modality result in the lowest limits of detection available in one compact instrument
Modular Design: purchase only what you need and easily upgrade later as experimental requirements expand
High Throughput: optimized for screening assays including 1536-well read mode

The Synergy 2 offers a small footprint, a robotic-friendly carrier for easy integration, and reads a 384-well plate in only 22 seconds.
Wide Range of Applications: with built-in temperature control, shaking, and optional dual reagent injector, virtually any microplate-based application is possible
- Screening assays (e.g. fluorescence polarization, TR-FRET, luminescence)
- Binding assays
- Ion channel assays
- Quantitative assays (DNA, protein)
- Kinetic assays
- Gene expression assays (GFP, Luciferase)
- ELISA assays
- Cell proliferation, Cytotoxicity
High Performance Screening Assays: The Synergy 2 is used in screening laboratories with all the major assay technologies such as:
- Cisbio International's HTRF® TR-FRET assays
- Invitrogen Corporation's LanthaScreen™, PolarScreen™, Omnia™, Z'-Lyte®, GeneBLAzer®, VSP ion channel assays
- AlphaScreen® and LANCE™ assays from Perkin Elmer, Inc.
- Promega Corporation’s Glo® and flash luminescent assays
Dual reagent injector for ion channel assays, flash luminescence and fast enzyme kinetics.
New Gen5™ Software: Synergy 2 is driven by the most powerful and efficient microplate control, data collection and analysis software available today.

Gen5™ Reader Control and Data Analysis Software

Models:

Synergy 2 is a modular instrument. Read modes and options are available as individual modules at the time of purchase, allowing you to make the best use of your budget. In addition, existing units can be upgraded later when your research requirements expand.

Synergy™ 2 Custom Configurations Contact BioTek for part #
Module	Description
S	Synergy 2 base model. Includes temperature control to 65ºC, Shaking, and Gen5™.
L	Luminescence
F	Fluorescence Intensity Top and Bottom. Includes Tungsten-Halogen light source.
P	Fluorescence Polarization. Requires F module.
T	Time-Resolved Fluorescence. Requires F module. Includes high energy DPR Xenon Flash Lamp.
A	UV-Visible Absorbance (monochromator-based). Includes SQ Xenon Flash Lamp.
D	Dual Reagent Dispenser

Red shifted PMT (900nm) option available instead of standard low noise PMT (700 nm).
3 mm and 1.5 mm diameter bottom fiber options available instead of standard 5 mm bottom fiber.

Specifications:

General
Detection method	Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa, Luminescence, UV-Visible Absorbance
Read method	End-point, kinetic, spectral scanning, well-area scanning
Microplate types	6- to 1536-well plates (luminescence 1-384) PCR plates Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control	4°C above ambient to 65°C
Shaking	Yes
Software	Gen5™ Included Control through USB or serial port
Automation	Yes
AlphaScreen/AlphaLISA
Light source	Tungsten Halogen Lamp
Sensitivity	100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate
Dynamic range	>6 decades
Reading speed	2 minutes typical for 96-well plate
Detection system	Ultra Low Noise PMT
Absorbance
Light source	SQ Xenon Flash
Wavelength selection	Monochromator
Wavelength range	200 - 999 nm, 1 nm increment
Bandpass	2.4 nm
Dynamic range	0 - 4.0 OD
Resolution	0.0001 OD
Pathlength correction	Yes
Monochromator wavelength accuracy	+/- 2 nm
Monochromator wavelength repeatability	+/- 0.2 nm
OD accuracy	< 1% at 2 OD typical < 3% at 3.0 OD typical
OD linearity	< 1% from 0 to 3.0 OD typical
OD repeatability	< 0.5% at 2.0 OD typical
Stray light	0.03% at 230 nm typical
Reading speed	96: 11 seconds 384: 22 seconds 1536: 42 seconds
Fluorescence Intensity
Sensitivity	Fluorescein 1 pM typical (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate) - Top Fluorescein 5 pM typical (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) - Bottom
Light source	Tungsten Halogen High Energy DPR Xenon Flash (optional)
Wavelength selection	Deep blocking bandpass filters/dichroic mirrors
Wavelength range	300 - 700 nm with tungsten lamp (900 nm option) 200 - 700 nm with xenon lamp (900 nm option)
Dynamic range	> 6 decades
Bandpass	Filter dependent
Detection system	PMT
Reading speed	96: 11 seconds 384: 22 seconds 1536: 42 seconds
Luminescence
Sensitivity	10 amol ATP typical (flash)
Wavelength range	300 - 700 nm
Dynamic range	> 6 decades
Detection system	Low noise PMT
Fluorescence Polarization
Light source	Tungsten Halogen High Energy DPR Xenon Flash (optional)
Sensitivity	3 mP at 1 nM fluorescein typical
Wavelength selection	Deep blocking filters/mirrors
Wavelength range	400 - 700 nm
Time-Resolved Fluorescence
Light source	High Energy DPR Xenon Flash
Sensitivity	Europium 60 fM typical (12 amol/well 96-well plate; 6 amol/well 384-well plate)
Wavelength selection	Deep blocking filters/mirrors
Wavelength range	200 - 700 nM (900 nm option)
Reagent Dispensers
Number	2 syringe pumps
Dispense volume	5 - 1000 µL in 1 µL increment
Dead volume	1.1 mL, 100 µL with backflush
Physical Characteristics
Power	250 Watts max.
Dimensions	17"W x 17.5"D x 14.5"H (43.5 x 44.5 x 37.3 cm)
Weight	60 lbs (27 kg)
Regulatory
Regulatory	For in vitro diagnostic use. All BioTek microplate instrumentation is CE and ETL marked. For details on approvals and standards compliance, please contact BioTek.