Detection > Hybrid Multi-Mode Microplate Readers
Synergy™ H4 with Hybrid Technology™ is a patent-pending multi-mode detector that combines the optical systems of Synergy Mx and Synergy 2 in one compact instrument. It is the ideal instrument for research and drug discovery applications when having to choose between flexibility and performance is not an option. The monochromator-based optics provides a high level of flexibility; any wavelength can be used from the low UV to the near infrared. The quadruple grating optical system, equipped with a variable bandpass selection system, is ideal for spectral scanning applications. The filter-based optics use dichroic mirrors for enhanced performance. This system is faster and more sensitive than the monochromator optics. A dual reagent dispenser option is available for inject and read assays such as flash luminescent assays and fluorescent ion channel assays. Additional read modes include: fluorescence polarization, time resolved fluorescence and AlphaScreen/AlphaLISA.
Watch this interview at SelectScience.tv with Marina Bruss from Analytica 2010 to learn more about the Synergy H4 Hybrid Multi-Mode Microplate Reader.
HTRF® and the HTRF logo are registered trademarks of Cisbio.
Transcreener® is a registered trademark of BellBrook Labs.
The LanthaScreen™ and GeneBLAzer® technologies from Invitrogen have been validated on BioTek instruments.
Synergy™ H4 is BioTek's second generation Hybrid reader. Like Synergy 4, it offers the combined benefits of filter-based and monochromator-based detection.
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Synergy™ H4 |
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| Part # | Absorbance | LUM | FI (Filters & Monos) | FP | TRF | Injectors | Software |
| H4MF | • | Gen5™ included | |||||
| H4MFD | • | • | |||||
| H4MFA | • | • | |||||
| H4MFAD | • | • | • | ||||
| H4MLF | • | • | |||||
| H4MLFD | • | • | • | ||||
| H4MLFA | • | • | • | ||||
| H4MLFAD | • | • | • | • | |||
| H4MLFPTA | • | • | • | • | • | ||
| H4MLFPTAD | • | • | • | • | • | • | |
| H4MFPT | • | • | • | ||||
| H4MFPTD | • | • | • | • | |||
| H4MLFPT | • | • | • | • | |||
| H4MLFPTD | • | • | • | • | • | ||
| H4MFPTA | • | • | • | • | |||
| H4MFPTAD | • | • | • | • | • | ||
| General | |
| Wavelength Selection | Hybrid Technology™ (patent-pending) Quadruple Monochromators and Filters / Dichroics |
| Detection method | Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance |
| Read method | End-point, kinetic, spectral scanning, well-area scanning |
| Microplate types | Monochromator system 1- to 384-well plates Filter system 1- to 1536-well plates PCR plates Compatible with Take3™ Multi-Volume Plate with 2 µL microspots |
| Temperature control | 4° C above ambient to 65° C ± 0.5° C at 37° C |
| Shaking | Yes |
| Software | Gen5™ Included Control through USB or serial port |
| Automation | Yes |
| AlphaScreen/AlphaLISA | |
| Light source | Tungsten Halogen Lamp |
| Sensitivity | 100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate |
| Dynamic range | >6 decades |
| Reading speed | 2 minutes typical for 96-well plate |
| Detection system | Ultra Low Noise PMT |
| Absorbance | |
| Light source | Xenon Flash Lamp |
| Wavelength selection | Monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increment |
| Bandpass | 2 nm (230-285 nm), 4 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | Yes |
| Monochromator wavelength accuracy | +/- 2 nm |
| Monochromator wavelength repeatability | +/- 0.2 nm |
| OD accuracy | < 1% at 2.0 OD typical < 3% at 3.0 OD typical |
| OD linearity | < 1% from 0 to 3.0 OD typical |
| OD repeatability | < 0.5% at 2.0 OD typical |
| Stray light | 0.03% at 230 nm typical |
| Reading speed | 96: 11 seconds 384: 22 seconds |
| Fluorescence Intensity | |
| Sensitivity | Monochromators: Fluorescein 2 pM typical (0.2 fmol/well 384-well plate) -Top Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) - Bottom Filters/mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate) |
| Light source | Tungsten Halogen Lamp High Energy Xenon Flash Lamp |
| Wavelength selection | Double grating monochromators (Top/Bottom) and, Deep blocking filters / dichroic mirrors (Top) |
| Wavelength range | Monochromators: 250 - 900 nm Filters: 200 - 700 nm (900 nm option) |
| Dynamic range | Monochromators: 5 decades Filters/mirrors: > 6 decades |
| Bandpass | Monochromators: variable 9, 13.5, 17, 20 nm Filters: filter-dependant, from 5 nm to >100 nm |
| Detection system | Red shifted PMT for monochromator system Low Noise PMT for filter-based system |
| Reading speed | 96: 11 seconds 384: 22 seconds 1536: 42 seconds |
| Luminescence | |
| Sensitivity | 10 amol ATP typical (flash) |
| Wavelength range | 300 - 700 nm |
| Dynamic range | > 6 decades |
| Fluorescence Polarization | |
| Light source | Tungsten Halogen |
| Sensitivity | 3 mP at 1 nM fluorescein typical |
| Wavelength selection | Deep blocking filters / dichroic mirrors (Top) |
| Wavelength range | 400 - 700 nm (300-900 nm option) |
| Time-Resolved Fluorescence | |
| Light source | High Energy Xenon Flash Lamp |
| Sensitivity | Europium 60 fM typical with filters (6 amol/well in 384-well plate) |
| Wavelength selection | Deep blocking filters / dichroic mirrors (Top) Double grating monochromator (Top / Bottom) |
| Wavelength range | Filters: 200 - 700 nm (900 nm option) Monochromators: 250 - 900 nm |
| Reagent Dispensers | |
| Number | 2 syringe pumps |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dead volume | 1.1 ml, 100 µL with back flush |
| Physical Characteristics | |
| Power | 250 Watts max. |
| Dimensions | 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm) |
| Weight | 78 lbs (35 kg) |
| Regulatory | |
| Regulatory | All BioTek microplate instrumentation is CE and ETL marked. For details on approvals and standards compliance, please contact BioTek. |