Synergy H1m Monochromator-Based Multi-Mode Microplate Reader
Synergy™ H1m is the most cost-effective monochromator-based multi-mode microplate reader on the market. Its monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate applications found in life science research laboratories. Synergy H1m can be turned into a high performance Synergy H1 Hybrid Multi-Mode Microplate Reader with the addition of a filter-based optical module. A dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).
- Fully monochromator-based with quadruple grating architecture
- Upgradeable to patent-pending Hybrid monochromator-filter optical system
- Compatible with Take3 Multi-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
- High performance fluorescence (top and bottom), absorbance and luminescence detection systems accommodate all life-science research microplate applications.
- Automated Z-height adjustment accommodates variable microplate heights and liquid levels with 100 μm resolution.
- Optional dual-reagent dispenser can be used for assays requiring precise timing such as ion channel assays or luminescent flash ATP assays.
- Comes with Gen5 software: reader control, advanced data analysis and flexible Excel export in one software package.
Model |
Synergy H1m |
|
Part # |
H1M |
H1MD |
Monochromator fluorescence |
• |
• |
Monochromator absorbance |
• |
• |
Luminescence |
• |
• |
Time-Resolved Fluorescence |
• * |
• * |
Dual reagent dispenser |
|
• |
Temperature control to 45°C |
• |
• |
Gen5 data analysis software |
• |
• |
* Secondary mode
| General | |
| Wavelength Selection | Quadruple Monochromators |
| Detection method | Fluorescence, Luminescence, UV-Visible Absorbance, TRF (secondary mode) |
| Read method | End-point, kinetic, spectral scanning, well-area scanning |
| Microplate types | 1- to 384-well plates Compatible with Take3 plate with 2 µL microspots |
| Maximum Plate Height | Absorbance: 0.8" (20.3 mm) Fluorescence & Luminescence: 0.89" (22.6 mm) |
| Temperature control | To 45°C +0.5°C at 37°C |
| Shaking | Yes |
| Software | Gen5™ reader control, advanced data analysis, Excel export Control through USB or serial port |
| Automation | Yes |
| Absorbance | |
| Light source | Xenon Flash Lamp |
| Wavelength selection | Monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increment |
| Bandpass | 4 nm (230-285 m), 8 nm (>285 m) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | Yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD typical <3% at 3.0 OD typical |
| OD linearity | <1% from 0 to 3.0 OD typical |
| OD repeatability | <0.5% at 2.0 OD typical |
| Stray light | 0.03% at 230 nm typical |
| Reading speed | 96: 11 seconds 384: 22 seconds |
| Fluorescence Intensity | |
| Sensitivity | Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) |
| Light source | Xenon Flash Lamp |
| Wavelength selection | Double grating monochromators (Top and Bottom) |
| Wavelength range | 250 – 700 nm (900 nm option) |
| Dynamic range | 5 decades |
| Detection system | Low noise PMT |
| Reading speed | 96: 11 seconds 384: 22 seconds |
| Luminescence | |
| Sensitivity | 20 amol ATP typical (flash) |
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Reagent Dispensers | |
| Dispense Precision | <2% at 50-200 µL |
| Dispense Accuracy | +1 µL or 2% |
| Number | 2 syringe pumps |
| Plate Geometry | 1- to 384-well microplates |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dead volume | 1 mL, 100 µL with back flush |
| Physical Characteristics | |
| Power | 130 Watts max |
| Dimensions | 15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm) |
| Weight | 50 lbs (22.5 kg) |
| Regulatory | |
| Regulatory | All BioTek microplate instrumentation is CE and ETL marked. For details on approvals and standards compliance, please contact BioTek |
Specifications are subject to change.
Standard (Included)
Gen5 Data Analysis Software
BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.
Optional
BioStack Microplate Stacker
The BioStack™ Microplate Stacker adds stacking capabilities to BioTek's Synergy™, PowerWave™, 405™ Touch, EL406™, ELx405™, MultiFlo™, MicroFlo™ Select, MicroFill™ and Precision™ product families.
Standard (Included) | ||
Cables | ||
| 75034 | Serial Cable, Modem (9-pin female to 9-pin male)  |
|
| 75108 | USB Cable (male A to male B) |
|
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
Optional | ||
Labware Holders | ||
| 7302030 | Cuvette Adapter |
|
Miscellaneous | ||
| 7270512 | BioCell Adapter Plate Assembly |
|
| 7272051 | BioCell 1 cm Quartz Vessel |
|
Take3 Micro-Volume Plate | ||
| TAKE3 | Take3 Micro-Volume Plate |
|
Test Plates & Reagents | ||
| 98155 | Sodium Fluorescein Powder (1 mg vial) |
|
| 7120779 | BioTek QC Check Solution #1 - Yellow (25 mL) |
|
| 7120782 | BioTek QC Check Solution #1 - Yellow (125 mL) |
|
| 7160006 | Glowell Adapter for Synergy Luminescence Tests |
|
| 7260522 | Absorbance Test Plate |
|
| 8030015 | Luminescence Test Plate |
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