Synergy 2 Alpha Microplate Reader
Synergy 2 Alpha is a microplate reader dedicated to the life science research applications of Alpha-based assay technologies (e.g. AlphaScreen®, AlphaLISA®, SureFire®). Its unique optical design makes it the most cost-effective Alpha reader on the market. In addition, Synergy 2 Alpha can be upgraded with other detection modes, such as UV-visible absorbance, fluorescence intensity, luminescence and more to accommodate a broad range of microplate applications. Whether you are looking for a dedicated Alpha reader, or a high-performance multimode reader, Synergy 2 Alpha has been designed to meet your needs.
Proven Performance on a Wide Range of Alpha Assays
AlphaLISA®
Human Insulin Concentration Curve. AlphaLISA signal plotted against insulin
concentration. A logistic 4-parameter best fi t applied to the data using Gen5™
Data Analysis Software. Limit of detection: 2.8 µIU/mL of insulin.
AlphaScreen®
AlphaScreen® cAMP Assay Data. cAMP concentrations from 5.0x10-6 M to
5.0x10-11 M, 25 µL/well in 384-well plate. Z' = 0.80. Signal/ Background = 22.5.
SureFire®
Phosphorylation of p-p38MAPK protein stimulated with EGF, Sorbitol, Anisomycin, and TNF-a measured at 0, 5', 10', 15', and 30'.
AlphaScreen®, AlphaLISA®, SureFire® are registered trademark of Perkin Elmer.
- Low cost / high performance Alpha optics combines Tungsten Halogen light source with ultra low noise PMT detector.
- Comes with pre-programmed protocol files, sample data, and detailed application notes
- Proven sensitivity: limit of detection <100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate, on par with dedicated laser-based detection systems
- Ideal for life science research applications of Alpha-based assays
- Upgradeable to Multi-Mode functionality: available detection modules include UV-Visible absorbance, fluorescence intensity, luminescence, fluorescence polarization, time-resolved fluorescence and dual reagent dispenser.
AlphaScreen®, AlphaLISA®, SureFire® are registered trademark of Perkin Elmer.
| General | |
| Detection method | AlphaScreen®, AlphaLISA®, SureFire® |
| Read method | End-point, kinetic, well-area scanning |
| Microplate types | 1- to 384-well plates |
| Maximum Plate Height | 1.20" (30.5 mm) |
| Temperature control | 4°C above ambient to 65°C ± 0.5°C at 37°C |
| Shaking | Yes |
| Software | Gen5™ included Control through USB or serial port |
| Automation | Yes |
| AlphaScreen/AlphaLISA | |
| Light source | Tungsten Halogen Lamp |
| Sensitivity | 100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate |
| Dynamic range | >6 decades |
| Reading speed | 2 minutes typical for 96-well plate |
| Detection system | Ultra Low Noise PMT |
| Physical Characteristics | |
| Dimensions | 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm) |
| Weight | 55 lbs (25 kg) |
| Regulatory | |
| Regulatory | For in vitro diagnostic use (Except in the European Union). All BioTek microplate instrumentation is CE and NRTL Safety Agency marked. For details on approvals and standards compliance, please contact BioTek |
Specifications are subject to change.
Standard (Included)
Gen5 Data Analysis Software
Optional
BioStack Microplate Stacker
Standard (Included) | ||
Cables | ||
| 75034 | Serial Cable, Modem (9-pin female to 9-pin male)  |
|
| 75108 | USB Cable (male A to male B) |
|
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
